Mikroorganismu biofilmu veidošanās uz kontaktlēcām
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Latvijas Universitāte
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Maģistra darbs ir uzrakstīts latviešu valodā uz 65 lappusēm.
Mērķis: Izstrādāt jaunu metodi S. epidermidis adhēzijas izvērtēšanai mīksto kontaktlēcu virsmā.
Darba uzdevumi: noteikt S.epidermidis adhēziju kontaktlēcās, izmantojot ica (+) un ica (-) S. epidermidis celmus.
Metode: 5 materiālu mīkstās kontaktlēcas mērcēja S.epidermidis šķīdumos, inkubēja, veidoja histoloģisko materiālu, izvērtēja mikroskopiski palielinājumā 10x100.
Rezultāti: Mikroorganismu adhēzija notiek pie visām kontaktlēcām. Jaunām kontaktlēcām vislielākā adhēzija novērota lotrafilcon B, lietotām methafilcon A materiālā. Mikroorganismu adhēzija ir atkarīga no kontaktlēcas hidrofobitātes un depozītu veidošanās. Ica (+) kultūras palielina adhēziju. Novērota biofilmu veidošanās sākuma stādija- baktēriju kolonijas.
Atslēgas vārdi: kontaktlēcas, S.epidermidis, adhēzija, biofilma.
The Master thesis is written in Latvian, contains 65 pages. Purpose: To develop a new method of S.epidermidis adhesion evaluation on soft contact lenses surface. Tasks: to determine the ability of S.epidermidis adherenece in contact lenses, using ica (+) and ica (-) S. epidermidis strain. Methods: lenses of 5 materials were soaked in S.epidermidis solutions, incubated, consisted of histological material, examined microscopically in magnification 10x100 Results: All contact lenses possess adhesion, in new contact lenses group lotrafilcon B possess the most, in worn – methafilcon A. Adhesion depended on hydrophobicity of contact lenses and tears deposit formation. Adhesion with ica (+) culture increases. Biofilm formation was observes in the early stages – bacterial colonies. Key words: contact lenses, S.epidermidis, adhezion, biofilm.
The Master thesis is written in Latvian, contains 65 pages. Purpose: To develop a new method of S.epidermidis adhesion evaluation on soft contact lenses surface. Tasks: to determine the ability of S.epidermidis adherenece in contact lenses, using ica (+) and ica (-) S. epidermidis strain. Methods: lenses of 5 materials were soaked in S.epidermidis solutions, incubated, consisted of histological material, examined microscopically in magnification 10x100 Results: All contact lenses possess adhesion, in new contact lenses group lotrafilcon B possess the most, in worn – methafilcon A. Adhesion depended on hydrophobicity of contact lenses and tears deposit formation. Adhesion with ica (+) culture increases. Biofilm formation was observes in the early stages – bacterial colonies. Key words: contact lenses, S.epidermidis, adhezion, biofilm.