| dc.contributor.advisor | Murovska, Modra | |
| dc.contributor.advisor | Žileviča, Aija | |
| dc.contributor.author | Ivanova, Karina | |
| dc.contributor.author | University of Latvia. August Kirchenstein Institute of Microbiology and Virolog | |
| dc.date.accessioned | 2019-01-16T19:51:45Z | |
| dc.date.available | 2019-01-16T19:51:45Z | |
| dc.date.issued | 2005 | |
| dc.identifier.uri | https://dspace.lu.lv/dspace/handle/7/46028 | |
| dc.description.tableofcontents | Contents:
Introduction 8
The aim and the objectives of the present study 9
Literature review 11
Biology o/HTLV-1 11
Structure of the virion 11
HTLV-1 life cycle 12
Genome structure and encoded proteins 14
The viral oncoprotein - Tax 19
Target cell and receptor 22
HTLV-1 associated diseases 23
Therapeutic nucleic acids and the mechanisms of their action 29
Antisense nucleic acids 29
RNA interference 30
Ribozymes 31
Transcription factor decoy 31
Construction of therapeutic nucleic acids' expression vectors 32
Choice of a promoter 32
Choice of the target 33
Studies in vitro 34
Experiments in vivo 36
Conclusions and future prospects 37
Materials and methods 38
Results 46
Establishment of experimental model of HTLV-1 infection in monolayer cell
line HOS 46
Construction of the plasmids carrying asRNA genes targeted at HTLV-1 LTR
U3 and pX region and Tax decoy HTLV-1 LTR U3 sequence 60
Investigation of the effect of HTLV-1 asRNA and Tax decoy plasmids on HTLV-1 replication in RaHOS cells 70
Investigation of the effect of HTLV-1 LTR U3 asRNA and Tax decoy plasmids
with inserted marker neo gene on virus replication in HTLV-1 transformed rabbit lymphoid cell line Ra-1 and human T-cell line MT-2 82
Discussion 91
Conclusions 97
Acnowledgements 98
References 99 | |
| dc.language.iso | eng | en_US |
| dc.rights | info:eu-repo/semantics/closedAccess | en_US |
| dc.subject | Research Subject Categories::MEDICINE::Microbiology, immunology, infectious diseases::Microbiology | en_US |
| dc.subject | Mikrobioloģija | en_US |
| dc.subject | Virusoloģija | en_US |
| dc.subject | Virology | en_US |
| dc.subject | Human T-cell lymphotropic virus type 1 | en_US |
| dc.subject | HTLV-1 | en_US |
| dc.subject | Limfotropā 1. tipa vīruss | en_US |
| dc.subject | Oncovirology | en_US |
| dc.subject | Medicīnas mikrobioloģija | en_US |
| dc.subject | HTLV-1 infekcija | en_US |
| dc.subject | Onkogēnie retrovīrusi | en_US |
| dc.title | Antisense and virus trans-activator decoy approaches of inhibiting replication of human T-cell lymphotropic virus type 1 (HTLV-1) | en_US |
| dc.type | info:eu-repo/semantics/doctoralThesis | en_US |
