Tumorigenitātes marķieru noteikšana pieaugušo cilmes šūnās in vitro
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Latvijas Universitāte
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Abstract
K-67 un telomerāzes reversā transkriptāze TERT ir olbaltumvielu dabas savienojumi, kuri ir iesaistīti ne tikai šūnu proliferācijas un novecošanas procesos, bet arī onkoģenēzē. Līdzšinējie pētījumi ļauj domāt, ka tos varētu izmantot kā marķierus šūnu tumorigenitātes prognozēšanai. Šī darba mērķis bija noskaidrot, vai un cik lielā mērā šo marķieru aktivitāte ir vērojama pieaugušu cilvēku dermas un kaulu smadzeņu mezenhimālajās cilmes šūnās, kā arī izpētīt iespējamās sakarības starp Ki-67 un TERT aktivitātes līmeni vai starp šo marķieru ekspresijas līmeni un citiem šūnas raksturojošiem parametriem.
Darbā izmantota imūnfluorescences metode. Iegūtie rezultāti liecina, ka Ki-67 apgriezti korelē ar šūnu pasāžas numuru un TERT aktivitātes līmeni. Turklāt konstatēts, ka tām dermas un kaulu smadzeņu mezenhimālo šūnu kultūrām, kuras neiekļāvās kopējās tendencēs, Ki-67 un TERT aktivitātes ziņā bija vērojama līdzība ar pārbaudītajām ļaundabīgo audzēju šūnu kultūrām.
K-67 and telomerase reverse transcriptase (TERT) are protein compounds involved not only in cell proliferation and senescence processes but also in oncogenesis. Available data from literature suggest that those compounds might be used as markers for the establishment of cell tumorigenicity prognosis in vivo. This work was aimed to identify whether and in what level the activity of the above mentioned markers can be observed in stem cells isolated from adult human derma and bone marrow and to investigate potential correlation between the acitivity of Ki-67 and TERT or between expression of these markers and other characteristics of cell culture. The immunofluorescence method was used. Results indicate inverse correlation of Ki-67 with cell line passage number and the level of TERT activity. Furthermore, it was noted that those derma and bone marrow cell lines which did not correspond to common trends, were quite similar in terms of Ki-67 and TERT activity to tested cancer cell lines.
K-67 and telomerase reverse transcriptase (TERT) are protein compounds involved not only in cell proliferation and senescence processes but also in oncogenesis. Available data from literature suggest that those compounds might be used as markers for the establishment of cell tumorigenicity prognosis in vivo. This work was aimed to identify whether and in what level the activity of the above mentioned markers can be observed in stem cells isolated from adult human derma and bone marrow and to investigate potential correlation between the acitivity of Ki-67 and TERT or between expression of these markers and other characteristics of cell culture. The immunofluorescence method was used. Results indicate inverse correlation of Ki-67 with cell line passage number and the level of TERT activity. Furthermore, it was noted that those derma and bone marrow cell lines which did not correspond to common trends, were quite similar in terms of Ki-67 and TERT activity to tested cancer cell lines.