Cilvēku dermas cilmes šūnu proliferācijas atšķirības
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Latvijas Universitāte
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Cilmes šūnu pētījumi šobrīd ir viena no daudzsološākajām biomedicīnas nozarēm. Taču, līdztekus daudzajiem atklājumiem, rodas arī daudzi jautājumi par cilmes šūnu izcelsmi, veidu, kā tās atrast un pavairot, to medicīnisko pielietojumu. Pieaudzis cilvēks var būt pats sev cilmes šūnu donors, tādējādi izvairoties no audu nesaderības, cilmes šūnu transplantācijas gadījumā. Tomēr individuālu cilvēku šūnu atšķirības in vitro ierobežo izolēto cilmes šūnu pētījumus, jo iegūtos rezultātus nevar vispārināt, lai izstrādātu zinātniski pamatotas šūnu audzēšanas vadlīnijas. Šajā pētījumā salīdzinājām dažādu donoru dermas cilmes šūnu reakciju uz vairākiem proliferācijas aģentiem. Secinājām, ka pastāv individuālu donoru šūnu atšķirības atbildes reakcijās pret FGF-2 un db-cAMF stimulāciju, bet visu donoru šūnu proliferāciju veicina FBS klātbūtne barotnē. Atšķiras arī bazālie šūnu cAMF līmeņi, bet tie nekorelē ar šūnu vairošanās spēju.
Stem cell investigation nowadays is one of the promising research branches. However, many questions about donor cell propagation in vitro still are not answered. One of them is inter-donor cell variability in vitro assays that makes problematic working-out of the guidelines how to expand cell cultures and how to use them in therapy. Adult human being may be stem cell donor for his own stem cell based therapy, but, unfortunately, these cells are not advanced for the fundamental research. Here, we tested inter donor cell differences in cell proliferation assays. We found that different donor dermal stem cells differentially respond to the FGF-2 and db-cAMP stimulation, but FBS always stimulated cell growth. Also, basal cAMP level differed in different donor cell cultures. However, correlation between cAMP level and response to proliferation stimulators was not revealed.
Stem cell investigation nowadays is one of the promising research branches. However, many questions about donor cell propagation in vitro still are not answered. One of them is inter-donor cell variability in vitro assays that makes problematic working-out of the guidelines how to expand cell cultures and how to use them in therapy. Adult human being may be stem cell donor for his own stem cell based therapy, but, unfortunately, these cells are not advanced for the fundamental research. Here, we tested inter donor cell differences in cell proliferation assays. We found that different donor dermal stem cells differentially respond to the FGF-2 and db-cAMP stimulation, but FBS always stimulated cell growth. Also, basal cAMP level differed in different donor cell cultures. However, correlation between cAMP level and response to proliferation stimulators was not revealed.