Retīnskābes ietekme uz TGFβ2 proteīna un mRNS sintēzi kardiovaskulārās sistēmas agrīnās attīstības stadijās in vito un in vitro
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Latvijas Universitāte
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Abstract
Promocijas darba “Retīnskābes ietekme uz TGFβ2 proteīna un mRNS sintēzi kardiovaskulārās
sistēmas agrīnās attīstības stadijās in vivo un in vitro” anotācija
A vitamīna un tā aktīvās formas retīnskābes (RA) deficīts izsauc smagus embrioģenēzes
traucējumus, kas saistīti ar kardiovaskulārās sistēmas attīstības defektiem. RA funkcijas agrīnās
ebrioģenēzes stadijās nav bijusi pētīta. Izpētot RA ietekmi uz TGFβ2 nonācām pie galveniem
secinājumiem: (i) vitamīna A deficīts putnu embrioģenēzes sākumposmā paaugstina TGFβ2
proteīna un mRNS uzkrāšanos sirdi veidojošajā rajonā; (ii) in vivo novērotos efektus iespējams
modelēt in vitro kultūrās, kas iegūtas no putnu sirdi veidojošā rajona un/vai cilvēka
kardiovaskulārām priekštečšūnām; (iii) TGFβ2 un RA mijiedarbība ir atkarīga no šūnu attīstības
stadijas un tipa.
Annotation of doctoral thesis “Effect of retinoic acid on TGFβ2 protein and mRNA synthesis during early cardiovascular development in vivo and in vitro” Deficiency of vitamin A and its active form retinoic acid (RA) during embryogenesis manifests in abnormalities of cardiovascular development. The function of RA at very early stages of heart development have not been studied. Examining the interaction between RA and TGFβ2 main conclusions are: (i) during early heart development the absence of vitamin A in the VAD quail embryo leads to an increased accumulation of TGFβ2 protein and its mRNA in the heart forming region during heart morphogenesis; (ii) the effects of RA observed in vivo can be duplicated in the in vitro systems of avian heart forming cells and human early cardiovascular progenitor cells; (iii) the interaction of TGFβ2 and retinoic acid is time and cell type dependent.
Annotation of doctoral thesis “Effect of retinoic acid on TGFβ2 protein and mRNA synthesis during early cardiovascular development in vivo and in vitro” Deficiency of vitamin A and its active form retinoic acid (RA) during embryogenesis manifests in abnormalities of cardiovascular development. The function of RA at very early stages of heart development have not been studied. Examining the interaction between RA and TGFβ2 main conclusions are: (i) during early heart development the absence of vitamin A in the VAD quail embryo leads to an increased accumulation of TGFβ2 protein and its mRNA in the heart forming region during heart morphogenesis; (ii) the effects of RA observed in vivo can be duplicated in the in vitro systems of avian heart forming cells and human early cardiovascular progenitor cells; (iii) the interaction of TGFβ2 and retinoic acid is time and cell type dependent.