Neirotrofo faktoru ietekme uz neiroektodermas marķieru ekspresiju ādas mezenhimālajās cilmes šūnās

Abstract

Kultivēšanas barotnes sastāvs ir faktors, kas stimulē cilmes šūnu diferenciāciju in vitro. Neirotrofie faktori virza diferenciāciju neiroektodermas šūnu virzienā. Bakalaura darba mērķis bija analizēt neirotrofo faktoru ietekmi uz neiroektodermas marķieru ekspresiju ādas mezenhimālajās cilmes šūnās (Ā-MCš). Neiroektodermas gēnu ekspresija Ā-MCš tika noteikta ar RT-PCR metodi pēc diferencēšanas trīs barotnēs. Tika izveidots 15 neiroektodermas gēnu praimeru panelis. Sox10, NES un TUBB3 gēni tika analizēti arī ar kvantitatīvo PCR (qPCR) metodi. RT-PCR rezultāti uzrādīja atšķirības Pax6 un S100β gēnu ekspresijā starp barotņu sastāviem. qPCR analīze parādīja, ka Švāna šūnu barotnē pieauga Sox10 ekspresija, neirodiferenciācijas barotnē pieauga TUBB3 ekspresija un NES ekspresija palielinājās gan neirodiferenciācijas, gan Švāna šūnu barotnēs.

The composition of cultivation medium is a factor that stimulates stem cell differentiation in vitro. Neurotrophic factors promote differentiation in the direction of neuroectodermal cells. The aim of bachelor work was to analyze the impact of neurotrophic factors on the expression of neuroectodermal markers in skin mesenchymal stem cells (S-MCs). Neuroectodermal gene expression in S-MCs was determined by RT-PCR method after differentiation in three mediums. The panel of 15 neuroectodermal gene primers was created. Sox10, NES, TUBB3 genes were also analyzed by quantitative PCR (qPCR) method. RT-PCR results showed differences in Pax6 and S100β gene expression between medium compositions. qPCR analysis showed that Sox10 expression was increased in Schwann cell medium, TUBB3 expression was increased in neural differentiation medium and NES expression was increased in neural differentiation and in Schwann cell media.