| dc.contributor.advisor | Riekstiņa, Una | en_US |
| dc.contributor.author | Saulīte, Līga | en_US |
| dc.contributor.other | Latvijas Universitāte. Bioloģijas fakultāte | en_US |
| dc.date.accessioned | 2015-07-05T01:08:18Z | |
| dc.date.available | 2015-07-05T01:08:18Z | |
| dc.date.issued | 2015 | en_US |
| dc.identifier.other | 48345 | en_US |
| dc.identifier.uri | https://dspace.lu.lv/dspace/handle/7/28556 | |
| dc.description.abstract | Ādas mezenhimālās cilmes šūnas (Ā-MCš), pakļautas specifiskiem faktoriem, spēj diferencēties par neironiem un glijas šūnām.
Maģistra darba mērķis bija analizēt Ā-MCš fenotipu pēc neirotrofo faktoru inducētas neirodiferenciācijas.
Neiroektodermas gēnu ekspresiju Ā-MCš analizēja ar qPCR, cilmes šūnu pluripotences un mezenhimālo marķieru ekspresiju ar plūsmas citometriju, Sigma-1 receptora ekspresiju (SigmaR1) ar qPCR un IF, kā arī BDNF sekrēciju ar ELISA. Ā-MCš fenotipu salīdzināja ar ReNcell CX šūnām.
Retīnskābes indukcijas barotnē novēroja nervu kores gēnu ekspresijas pieaugumu. Cilmes šūnu marķieru ekspresija pēc diferenciācijas kritās. Mezenhimālo marķieru ekspresija samazinājās vienā no testētajām donoru šūnu līnijām. SigmaR1 visaugstāk ekspresēja nediferencētas Ā-MCš. Švāna šūnu barotnē šūnas pastiprināti sekretēja BDNF. | en_US |
| dc.description.abstract | Skin mesenchymal stem cells (S-MSc) in the presence of specific growth factors may differentiate to neurons and glial cells.
The aim of the master thesis was to analyse the phenotype of S-MSc after neurotrophic factor induced neurodifferentiation.
S-MSc were analysed for neuroectodermal gene expression with qPCR, for stem cell pluripotency and mesenchymal markers with flow cytometry, for Sigma-1 receptor (SigmaR1) expression and for BDNF secretion with ELISA. The phenotype of S-MSc was compared to ReNcell CX cell line.
The increase of neural gene expression in S-MSc was observed in retinoic acid induction medium. Stem cell marker expression was decreased after differentiation. Mesenchymal stem cell marker expression was decreased in one of the three tested donor cell lines. The highest expression of SigmaR1 was observed in undifferentiated S-MSc. Cells cultivated in Schwann cells medium enhanced the secretion of BDNF. | en_US |
| dc.language.iso | N/A | en_US |
| dc.publisher | Latvijas Universitāte | en_US |
| dc.rights | info:eu-repo/semantics/openAccess | en_US |
| dc.subject | Bioloģija | en_US |
| dc.subject | mezenhimālās cilmes šūnas | en_US |
| dc.subject | neiroektoderma | en_US |
| dc.subject | Sigma-1 receptors | en_US |
| dc.subject | BDNF | en_US |
| dc.subject | ReNcell CX | en_US |
| dc.title | Ādas mezenhimālo cilmes šūnu fenotipa raksturojums pēc neirotrofo faktoru inducētas neirodiferenciācijas | en_US |
| dc.title.alternative | Phenotypic characterization of skin mesenchymal stem cells fallowing the neurotrophic factor induced neurodifferentiation | en_US |
| dc.type | info:eu-repo/semantics/masterThesis | en_US |
